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antibodies against cpt1a  (Proteintech)


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    Proteintech antibodies against cpt1a
    Antibodies Against Cpt1a, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 606 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cpt1a+antibodies/CPT1A+Antibody/pm42911364-74-21-26
    Average 96 stars, based on 606 article reviews
    antibodies against cpt1a - by Bioz Stars, 2026-11
    96/100 stars

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    Western Blot:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Infection:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    shRNA:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Gene Expression:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Microarray:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Transfection:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Plasmid Preparation:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Control:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.

    Expressing:

    Article Title: Mechanistic insights into the PAI-1 inhibitor PAItrap3: enhancing lipid metabolism in adipose tissue of diabetic db/db mice
    Article Snippet: ELISA kits for mouse insulin (INS), glycated hemoglobin (GHb), nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China).κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), triglycerides (TG), total cholesterol (TC), and free fatty acids (FFAs) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., (Quanzhou, China). ... Antibodies for Parkin and LC3B were obtained from Cell Signaling Technology (catalog numbers: 2132S and 83506S, MA, United States); P62 antibody was from Abcam (catalog number: ab56416, Cambridge, MA, United States); PPARGC1B, ACADM, and CPT1A antibodies were from Proteintech (catalog numbers: 22378-1-AP, 55210-1-AP, and 15184-1-AP, Wuhan, China); FASN, PGC1α, and HSL antibodies were from ABclonal (catalog numbers: A19050, A12348, and A24689, Wuhan, China); and β-actin antibody was from Affinity Biosciences (catalog number: T0022, Jiangsu, China).. HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from Vazyme (Nanjing, China).HRP-conjugated anti-rabbit IgG and anti-mouse IgG secondary antibodies were purchased from Boster Biological Technology (Wuhan, China). qRT-PCR kits, reverse transcription kits, and RNA extraction kits were obtained from..

    Article Title: FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
    Article Snippet: Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).Cell lysate concentrations were measured by the Bradford assay (ThermoFisher Scientific), and equal amounts of proteins were loaded onto an SDS-polyacrylamide gel, separated by electrophoresis and blotted onto a nitrocellulose membrane (Millipore).. Rabbit FDXR (15584-1-AP) and CPT1A antibodies (15184-1-AP) were purchased from Proteintech.. Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.Mouse Vinculin (V9131) and mouse Flag (F3165) antibodies were purchased from Sigma−Aldrich.



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    Proteintech cpt1a
    β‐OHB promotes <t>CPT1A</t> Kbhb modification at lysine 344 via P300. (A) Relative levels of spinal cord β‐OHB in sham and SCI mice at 14 dpi ( n = 5 per group). (B) Kbhb analysis of spinal cord tissues from sham and SCI mice at 14 dpi. (C) The specific CPT1A peptide segments were identified in the Kbhb complex obtained by immunoprecipitation from astrocytes. (D) CPT1A was immunoprecipitated from spinal cord tissues under specific conditions, and its Kbhb modification level was detected using a Kbhb antibody. (E) Primary astrocytes treated with a concentration gradient of β‐OHB were subjected to immunoprecipitation with a CPT1A antibody, followed by detection of CPT1A Kbhb modification levels using a Kbhb antibody. (F) Primary astrocytes were transfected with either an empty vector or constructing HA‐GCN5, HA‐PCAF, HA‐CBP, or HA‐P300. The Kbhb modification was further analyzed. (G) Astrocytes transfected with or without sh p300 were treated with 10 mM β‐OHB for 24 h. Cell lysates were then subjected to immunoprecipitation with an <t>anti‐CPT1A</t> antibody, followed by detecting CPT1A Kbhb levels. (H) Schematic diagram of the CPT1A molecular structure. (I) Schematic diagram of the lysine mutation sites in CPT1A protein. (J) HEK293T cells transfected with Flag‐CPT1A (WT or mutants) were treated with 10 mM β‐OHB for 24 h, followed by immunoprecipitation and immunoblotting to detect CPT1A Kbhb modification levels. (K) HEK293T cells co‐transfected with Flag‐CPT1A (WT or K344R) and HA‐P300, followed by immunoprecipitation with a Flag antibody and immunoblotting to detect the Kbhb modification levels of CPT1A. Data were analyzed using a two‐tailed unpaired Student's t ‐test (A).
    Cpt1a, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    carnitine palmitoyltransferase 1a  (Proteintech)
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    Proteintech carnitine palmitoyltransferase 1a
    β‐OHB promotes <t>CPT1A</t> Kbhb modification at lysine 344 via P300. (A) Relative levels of spinal cord β‐OHB in sham and SCI mice at 14 dpi ( n = 5 per group). (B) Kbhb analysis of spinal cord tissues from sham and SCI mice at 14 dpi. (C) The specific CPT1A peptide segments were identified in the Kbhb complex obtained by immunoprecipitation from astrocytes. (D) CPT1A was immunoprecipitated from spinal cord tissues under specific conditions, and its Kbhb modification level was detected using a Kbhb antibody. (E) Primary astrocytes treated with a concentration gradient of β‐OHB were subjected to immunoprecipitation with a CPT1A antibody, followed by detection of CPT1A Kbhb modification levels using a Kbhb antibody. (F) Primary astrocytes were transfected with either an empty vector or constructing HA‐GCN5, HA‐PCAF, HA‐CBP, or HA‐P300. The Kbhb modification was further analyzed. (G) Astrocytes transfected with or without sh p300 were treated with 10 mM β‐OHB for 24 h. Cell lysates were then subjected to immunoprecipitation with an <t>anti‐CPT1A</t> antibody, followed by detecting CPT1A Kbhb levels. (H) Schematic diagram of the CPT1A molecular structure. (I) Schematic diagram of the lysine mutation sites in CPT1A protein. (J) HEK293T cells transfected with Flag‐CPT1A (WT or mutants) were treated with 10 mM β‐OHB for 24 h, followed by immunoprecipitation and immunoblotting to detect CPT1A Kbhb modification levels. (K) HEK293T cells co‐transfected with Flag‐CPT1A (WT or K344R) and HA‐P300, followed by immunoprecipitation with a Flag antibody and immunoblotting to detect the Kbhb modification levels of CPT1A. Data were analyzed using a two‐tailed unpaired Student's t ‐test (A).
    Carnitine Palmitoyltransferase 1a, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibodies against cpt1a  (ABclonal Biotechnology)
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    β‐OHB promotes <t>CPT1A</t> Kbhb modification at lysine 344 via P300. (A) Relative levels of spinal cord β‐OHB in sham and SCI mice at 14 dpi ( n = 5 per group). (B) Kbhb analysis of spinal cord tissues from sham and SCI mice at 14 dpi. (C) The specific CPT1A peptide segments were identified in the Kbhb complex obtained by immunoprecipitation from astrocytes. (D) CPT1A was immunoprecipitated from spinal cord tissues under specific conditions, and its Kbhb modification level was detected using a Kbhb antibody. (E) Primary astrocytes treated with a concentration gradient of β‐OHB were subjected to immunoprecipitation with a CPT1A antibody, followed by detection of CPT1A Kbhb modification levels using a Kbhb antibody. (F) Primary astrocytes were transfected with either an empty vector or constructing HA‐GCN5, HA‐PCAF, HA‐CBP, or HA‐P300. The Kbhb modification was further analyzed. (G) Astrocytes transfected with or without sh p300 were treated with 10 mM β‐OHB for 24 h. Cell lysates were then subjected to immunoprecipitation with an <t>anti‐CPT1A</t> antibody, followed by detecting CPT1A Kbhb levels. (H) Schematic diagram of the CPT1A molecular structure. (I) Schematic diagram of the lysine mutation sites in CPT1A protein. (J) HEK293T cells transfected with Flag‐CPT1A (WT or mutants) were treated with 10 mM β‐OHB for 24 h, followed by immunoprecipitation and immunoblotting to detect CPT1A Kbhb modification levels. (K) HEK293T cells co‐transfected with Flag‐CPT1A (WT or K344R) and HA‐P300, followed by immunoprecipitation with a Flag antibody and immunoblotting to detect the Kbhb modification levels of CPT1A. Data were analyzed using a two‐tailed unpaired Student's t ‐test (A).
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    antibodies recognizing cpt1a  (ABclonal Biotechnology)
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    β‐OHB promotes <t>CPT1A</t> Kbhb modification at lysine 344 via P300. (A) Relative levels of spinal cord β‐OHB in sham and SCI mice at 14 dpi ( n = 5 per group). (B) Kbhb analysis of spinal cord tissues from sham and SCI mice at 14 dpi. (C) The specific CPT1A peptide segments were identified in the Kbhb complex obtained by immunoprecipitation from astrocytes. (D) CPT1A was immunoprecipitated from spinal cord tissues under specific conditions, and its Kbhb modification level was detected using a Kbhb antibody. (E) Primary astrocytes treated with a concentration gradient of β‐OHB were subjected to immunoprecipitation with a CPT1A antibody, followed by detection of CPT1A Kbhb modification levels using a Kbhb antibody. (F) Primary astrocytes were transfected with either an empty vector or constructing HA‐GCN5, HA‐PCAF, HA‐CBP, or HA‐P300. The Kbhb modification was further analyzed. (G) Astrocytes transfected with or without sh p300 were treated with 10 mM β‐OHB for 24 h. Cell lysates were then subjected to immunoprecipitation with an <t>anti‐CPT1A</t> antibody, followed by detecting CPT1A Kbhb levels. (H) Schematic diagram of the CPT1A molecular structure. (I) Schematic diagram of the lysine mutation sites in CPT1A protein. (J) HEK293T cells transfected with Flag‐CPT1A (WT or mutants) were treated with 10 mM β‐OHB for 24 h, followed by immunoprecipitation and immunoblotting to detect CPT1A Kbhb modification levels. (K) HEK293T cells co‐transfected with Flag‐CPT1A (WT or K344R) and HA‐P300, followed by immunoprecipitation with a Flag antibody and immunoblotting to detect the Kbhb modification levels of CPT1A. Data were analyzed using a two‐tailed unpaired Student's t ‐test (A).
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    Image Search Results


    β‐OHB promotes CPT1A Kbhb modification at lysine 344 via P300. (A) Relative levels of spinal cord β‐OHB in sham and SCI mice at 14 dpi ( n = 5 per group). (B) Kbhb analysis of spinal cord tissues from sham and SCI mice at 14 dpi. (C) The specific CPT1A peptide segments were identified in the Kbhb complex obtained by immunoprecipitation from astrocytes. (D) CPT1A was immunoprecipitated from spinal cord tissues under specific conditions, and its Kbhb modification level was detected using a Kbhb antibody. (E) Primary astrocytes treated with a concentration gradient of β‐OHB were subjected to immunoprecipitation with a CPT1A antibody, followed by detection of CPT1A Kbhb modification levels using a Kbhb antibody. (F) Primary astrocytes were transfected with either an empty vector or constructing HA‐GCN5, HA‐PCAF, HA‐CBP, or HA‐P300. The Kbhb modification was further analyzed. (G) Astrocytes transfected with or without sh p300 were treated with 10 mM β‐OHB for 24 h. Cell lysates were then subjected to immunoprecipitation with an anti‐CPT1A antibody, followed by detecting CPT1A Kbhb levels. (H) Schematic diagram of the CPT1A molecular structure. (I) Schematic diagram of the lysine mutation sites in CPT1A protein. (J) HEK293T cells transfected with Flag‐CPT1A (WT or mutants) were treated with 10 mM β‐OHB for 24 h, followed by immunoprecipitation and immunoblotting to detect CPT1A Kbhb modification levels. (K) HEK293T cells co‐transfected with Flag‐CPT1A (WT or K344R) and HA‐P300, followed by immunoprecipitation with a Flag antibody and immunoblotting to detect the Kbhb modification levels of CPT1A. Data were analyzed using a two‐tailed unpaired Student's t ‐test (A).

    Journal: Advanced Science

    Article Title: Astrocytic Ketogenesis Protects Central Nervous System Injury via CPT1A Stabilization and Epigenetic Regulation

    doi: 10.1002/advs.78393

    Figure Lengend Snippet: β‐OHB promotes CPT1A Kbhb modification at lysine 344 via P300. (A) Relative levels of spinal cord β‐OHB in sham and SCI mice at 14 dpi ( n = 5 per group). (B) Kbhb analysis of spinal cord tissues from sham and SCI mice at 14 dpi. (C) The specific CPT1A peptide segments were identified in the Kbhb complex obtained by immunoprecipitation from astrocytes. (D) CPT1A was immunoprecipitated from spinal cord tissues under specific conditions, and its Kbhb modification level was detected using a Kbhb antibody. (E) Primary astrocytes treated with a concentration gradient of β‐OHB were subjected to immunoprecipitation with a CPT1A antibody, followed by detection of CPT1A Kbhb modification levels using a Kbhb antibody. (F) Primary astrocytes were transfected with either an empty vector or constructing HA‐GCN5, HA‐PCAF, HA‐CBP, or HA‐P300. The Kbhb modification was further analyzed. (G) Astrocytes transfected with or without sh p300 were treated with 10 mM β‐OHB for 24 h. Cell lysates were then subjected to immunoprecipitation with an anti‐CPT1A antibody, followed by detecting CPT1A Kbhb levels. (H) Schematic diagram of the CPT1A molecular structure. (I) Schematic diagram of the lysine mutation sites in CPT1A protein. (J) HEK293T cells transfected with Flag‐CPT1A (WT or mutants) were treated with 10 mM β‐OHB for 24 h, followed by immunoprecipitation and immunoblotting to detect CPT1A Kbhb modification levels. (K) HEK293T cells co‐transfected with Flag‐CPT1A (WT or K344R) and HA‐P300, followed by immunoprecipitation with a Flag antibody and immunoblotting to detect the Kbhb modification levels of CPT1A. Data were analyzed using a two‐tailed unpaired Student's t ‐test (A).

    Article Snippet: The primary and secondary antibodies included mouse/rabbit anti‐GFAP (GB12096, GB11096, Servicebio), rabbit anti‐CD68 (28058‐1‐AP, Proteintech), mouse anti‐CPT1A (66039‐1‐Ig, Proteintech), mouse anti‐NeuN (ab104224, Abcam), rabbit anti‐NeuN (GB11138, Servicebio), CoraLite 594‐conjugated GFAP antibody (CL594‐260190, Proteintech), mouse anti‐Perilipin‐2 (15294‐1‐AP, Proteintech), mouse anti‐SOX9 (67439‐1‐Ig, Proteintech), rabbit anti 5‐HT (20080, Immunostar), Mouse anti‐ChAT (66816‐1‐Ig, Proteintech), CoraLite 647‐conjugated rabbit polyclonal anti‐Syn (CL647‐17785, Proteintech), rabbit anti‐H3K9bhb (PTM‐1250RM, PTM Bio), Alexa Fluor488‐conjugated goat anti‐rabbit IgG (111‐545‐003, Jackson ImmunoResearch), Alexa Fluor594‐conjugated goat anti‐mouse IgG (115‐585‐003, Jackson ImmunoResearch), and Alexa Fluor647‐conjugated goat anti‐mouse IgG (115‐605‐003, Jackson ImmunoResearch).

    Techniques: Modification, Analysis, Immunoprecipitation, Concentration Assay, Transfection, Plasmid Preparation, Mutagenesis, Western Blot, Two Tailed Test

    Astrocytic ketogenesis promotes wound repair and lipid metabolic reprogramming, forming a positive feedback loop via H3K9bhb‐dependent epigenetic remodeling. (A) Western blot analysis of histone Kbhb modifications in mouse spinal cord tissues ( n = 3 per group). (B) Immunofluorescence analysis of H3K9bhb expression levels in spinal cord astrocytes of sham and SCI mice at 14 dpi ( n = 10 per group). Scale bar = 100 µm. (C) DeepTools visualization of ATAC‐seq chromatin accessibility regions. (D) Motif analysis of genes with upregulated ATAC‐seq chromatin accessibility. (E) DeepTools visualization of H3K9bhb binding density. (F) GO enrichment analysis of DEGs characterized by both increased chromatin accessibility and enhanced H3K9bhb enrichment. (G) Genome browser analysis of ATAC‐seq and CUT&Tag signals for Acat2 , Acadl , Acaa1a , Hmgcl , Mki67 , Pcna , Myc , and Tgfb3 . The red rectangle highlights the different peak region of the relevant genes. (H) ChIP‐qPCR analysis of the promoters of the indicated genes using anti‐H3K9bhb antibodies from sham and SCI mice at 14 dpi ( n = 5 per group). (I) mRNA levels of specified genes from sham and SCI mice at 14 dpi ( n = 5 per group). (J) Quantitative analysis of β‐OHB levels in astrocytes of the indicated groups ( n = 6 per group). (K) Western blot analysis of Kbhb and H3K9bhb levels in astrocytes of the indicated groups ( n = 5 per group). (L) RT‐qPCR analysis of mRNA levels of relevant genes in astrocytes of the indicated groups ( n = 5 per group). Data were analyzed using a two‐tailed unpaired Student's t ‐test (A, B, H, I) and one‐way ANOVA followed by post hoc Bonferroni correction (J‐L).

    Journal: Advanced Science

    Article Title: Astrocytic Ketogenesis Protects Central Nervous System Injury via CPT1A Stabilization and Epigenetic Regulation

    doi: 10.1002/advs.78393

    Figure Lengend Snippet: Astrocytic ketogenesis promotes wound repair and lipid metabolic reprogramming, forming a positive feedback loop via H3K9bhb‐dependent epigenetic remodeling. (A) Western blot analysis of histone Kbhb modifications in mouse spinal cord tissues ( n = 3 per group). (B) Immunofluorescence analysis of H3K9bhb expression levels in spinal cord astrocytes of sham and SCI mice at 14 dpi ( n = 10 per group). Scale bar = 100 µm. (C) DeepTools visualization of ATAC‐seq chromatin accessibility regions. (D) Motif analysis of genes with upregulated ATAC‐seq chromatin accessibility. (E) DeepTools visualization of H3K9bhb binding density. (F) GO enrichment analysis of DEGs characterized by both increased chromatin accessibility and enhanced H3K9bhb enrichment. (G) Genome browser analysis of ATAC‐seq and CUT&Tag signals for Acat2 , Acadl , Acaa1a , Hmgcl , Mki67 , Pcna , Myc , and Tgfb3 . The red rectangle highlights the different peak region of the relevant genes. (H) ChIP‐qPCR analysis of the promoters of the indicated genes using anti‐H3K9bhb antibodies from sham and SCI mice at 14 dpi ( n = 5 per group). (I) mRNA levels of specified genes from sham and SCI mice at 14 dpi ( n = 5 per group). (J) Quantitative analysis of β‐OHB levels in astrocytes of the indicated groups ( n = 6 per group). (K) Western blot analysis of Kbhb and H3K9bhb levels in astrocytes of the indicated groups ( n = 5 per group). (L) RT‐qPCR analysis of mRNA levels of relevant genes in astrocytes of the indicated groups ( n = 5 per group). Data were analyzed using a two‐tailed unpaired Student's t ‐test (A, B, H, I) and one‐way ANOVA followed by post hoc Bonferroni correction (J‐L).

    Article Snippet: The primary and secondary antibodies included mouse/rabbit anti‐GFAP (GB12096, GB11096, Servicebio), rabbit anti‐CD68 (28058‐1‐AP, Proteintech), mouse anti‐CPT1A (66039‐1‐Ig, Proteintech), mouse anti‐NeuN (ab104224, Abcam), rabbit anti‐NeuN (GB11138, Servicebio), CoraLite 594‐conjugated GFAP antibody (CL594‐260190, Proteintech), mouse anti‐Perilipin‐2 (15294‐1‐AP, Proteintech), mouse anti‐SOX9 (67439‐1‐Ig, Proteintech), rabbit anti 5‐HT (20080, Immunostar), Mouse anti‐ChAT (66816‐1‐Ig, Proteintech), CoraLite 647‐conjugated rabbit polyclonal anti‐Syn (CL647‐17785, Proteintech), rabbit anti‐H3K9bhb (PTM‐1250RM, PTM Bio), Alexa Fluor488‐conjugated goat anti‐rabbit IgG (111‐545‐003, Jackson ImmunoResearch), Alexa Fluor594‐conjugated goat anti‐mouse IgG (115‐585‐003, Jackson ImmunoResearch), and Alexa Fluor647‐conjugated goat anti‐mouse IgG (115‐605‐003, Jackson ImmunoResearch).

    Techniques: Western Blot, Analysis, Immunofluorescence, Expressing, Binding Assay, ChIP-qPCR, Quantitative RT-PCR, Two Tailed Test

    High levels of β‐OHB in the CSF of SCI patients indicate better tissue preservation and functional recovery. (A) Schematic illustrating the clinical study design for control and SCI patients. (B) Measurement of β‐OHB content in CSF (healthy controls, n = 20; SCI patients, n = 45). (C) Analysis of CSF β‐OHB levels based on motor recovery in ASIA grade A/B SCI patients (ASIA grade improvement, n = 8; No ASIA grade improvement, n = 17). (D) Representative MRI T2‐weighted images from healthy controls and SCI patients, and correlation analysis between hyperintensity areas in T2‐weighted MRI and β‐OHB levels in SCI patients ( n = 45). (E) Representative DTI images of SCI patients. (F) Correlation analysis between FA values and β‐OHB levels in SCI patients ( n = 45). (G,H) Correlation analysis between CSF GFAP (G)/NF‐L (H) levels and β‐OHB levels in SCI patients ( n = 45). (I) Schematic presentation of the study. Data were analyzed using a two‐tailed unpaired Student's t ‐test (B, C) and Pearson correlation analysis (D, F–H).

    Journal: Advanced Science

    Article Title: Astrocytic Ketogenesis Protects Central Nervous System Injury via CPT1A Stabilization and Epigenetic Regulation

    doi: 10.1002/advs.78393

    Figure Lengend Snippet: High levels of β‐OHB in the CSF of SCI patients indicate better tissue preservation and functional recovery. (A) Schematic illustrating the clinical study design for control and SCI patients. (B) Measurement of β‐OHB content in CSF (healthy controls, n = 20; SCI patients, n = 45). (C) Analysis of CSF β‐OHB levels based on motor recovery in ASIA grade A/B SCI patients (ASIA grade improvement, n = 8; No ASIA grade improvement, n = 17). (D) Representative MRI T2‐weighted images from healthy controls and SCI patients, and correlation analysis between hyperintensity areas in T2‐weighted MRI and β‐OHB levels in SCI patients ( n = 45). (E) Representative DTI images of SCI patients. (F) Correlation analysis between FA values and β‐OHB levels in SCI patients ( n = 45). (G,H) Correlation analysis between CSF GFAP (G)/NF‐L (H) levels and β‐OHB levels in SCI patients ( n = 45). (I) Schematic presentation of the study. Data were analyzed using a two‐tailed unpaired Student's t ‐test (B, C) and Pearson correlation analysis (D, F–H).

    Article Snippet: The primary and secondary antibodies included mouse/rabbit anti‐GFAP (GB12096, GB11096, Servicebio), rabbit anti‐CD68 (28058‐1‐AP, Proteintech), mouse anti‐CPT1A (66039‐1‐Ig, Proteintech), mouse anti‐NeuN (ab104224, Abcam), rabbit anti‐NeuN (GB11138, Servicebio), CoraLite 594‐conjugated GFAP antibody (CL594‐260190, Proteintech), mouse anti‐Perilipin‐2 (15294‐1‐AP, Proteintech), mouse anti‐SOX9 (67439‐1‐Ig, Proteintech), rabbit anti 5‐HT (20080, Immunostar), Mouse anti‐ChAT (66816‐1‐Ig, Proteintech), CoraLite 647‐conjugated rabbit polyclonal anti‐Syn (CL647‐17785, Proteintech), rabbit anti‐H3K9bhb (PTM‐1250RM, PTM Bio), Alexa Fluor488‐conjugated goat anti‐rabbit IgG (111‐545‐003, Jackson ImmunoResearch), Alexa Fluor594‐conjugated goat anti‐mouse IgG (115‐585‐003, Jackson ImmunoResearch), and Alexa Fluor647‐conjugated goat anti‐mouse IgG (115‐605‐003, Jackson ImmunoResearch).

    Techniques: Tissue, Preserving, Functional Assay, Control, Analysis, Two Tailed Test